penetration hyphal growth in rice cells

To investigate the mitotic dynamics of an appressorium, we used time-lapse confocal imaging of a fluorescence-based mitotic reporter strain of Magnaporthe oryzae. We present evidence that: (i) appressoria remain viable and mitotically active after host penetration, (ii) appressorial mitosis, like invasive hyphal mitosis, is semi-closed, (iii) sister chromatids separate within the appressorium, (iv) a mitotic appressorial nucleus undergoes extreme constriction and elongation as it migrates through the penetration peg in a manner analogous to mitosis during cell-to-cell movement of invasive hyphae. These results provide new insight into the potential roles of the appressorium after host penetration and highlight the unique mitotic dynamics during rice blast infection.